Sodium Flux Ratio in Na/K Pump-Channels Opened by Palytoxin

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Sodium Flux Ratio in Na/K Pump-Channels Opened by Palytoxin

Palytoxin binds to Na(+)/K(+) pumps in the plasma membrane of animal cells and opens an electrodiffusive cation pathway through the pumps. We investigated properties of the palytoxin-opened channels by recording macroscopic and microscopic currents in cell bodies of neurons from the giant fiber lobe, and by simultaneously measuring net current and (22)Na(+) efflux in voltage-clamped, internally...

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Large Diameter of Palytoxin-induced Na/K Pump Channels and Modulation of Palytoxin Interaction by Na/K Pump Ligands

Palytoxin binds to Na/K pumps to generate nonselective cation channels whose pore likely comprises at least part of the pump's ion translocation pathway. We systematically analyzed palytoxin's interactions with native human Na/K pumps in outside-out patches from HEK293 cells over a broad range of ionic and nucleotide conditions, and with or without cardiotonic steroids. With 5 mM internal (pipe...

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Palytoxin Inhibits the Sodium-Potassium Pump - An Investigation of an Electrophysiological Model Using Probabilistic Model Checking

Automatic veri cation techniques such as Probabilistic Model Checking (PMC) have been successfully applied in the speci cation and analysis of stochastic systems. Some biological systems show these characteristics, allowing PMC usage in unexpected elds. We present and analyze a probabilistic model for palytoxin toxin (PTX) e ects on cell transport systems, structures which exchange ions across ...

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Na+/K+-pump ligands modulate gating of palytoxin-induced ion channels.

The Na+/K+ pump is a ubiquitous P-type ATPase that binds three cytoplasmic Na+ ions deep within its core where they are temporarily occluded before being released to the extracellular surface. The 3Na+/2K+ -exchange transport cycle is completed when two extracellular K+ ions bind and become temporarily occluded within the protein and subsequently released to the cytoplasm. Coupling of Na+ -ion ...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 2007

ISSN: 1540-7748,0022-1295

DOI: 10.1085/jgp.200709770